Active Recombinant Human STAMBP, GST-tagged

• Cat.No.: STAMBP-167H
• Product Overview: Recombinant human STAMBP (amino acid residues 252-424), fused with N-terminal GST, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 252-424 a.a.
• Description: AMSH is a member of the JAB1/MPN/Mov34 metalloenzyme (JAMM) family and cloning of the human gene was first described by Tanaka et al. (1999). AMSH and AMSH-LP share 54% identity and 75% sequence similarity in their JAMM domain. It is known that both proteins act as regulators of free ubiquitin in the cell, bind clathrin, and contain a putative nuclear localization signal and a microtubule interacting and transport (MIT) domain. AMSH also contains a Src homology 3 (SH3) domain, facilitating its interaction with signal transducing adaptor molecule (STAM), while this functional SH3-binding motif is lost in AMSH-LP (Davies, et al., 2011).
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: The activity of GST-AMSH CD(252-424) was validated by the monitoring of mono-ubiquitin generation as a result of the enzyme catalysed cleavage of K63-linked di-ubiquitin. Incubation of the substrate in the presence or absence of GST-AMSH CD(252-424) was compared confirming the deubiquitylating activity of GST-AMSH CD(252-424).
• Molecular Mass: 47.1 kDa
• Purity: >89% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: STAMBP STAM binding protein [ Homo sapiens ]
• Official Symbol: STAMBP
• Synonyms: STAMBP; STAM binding protein; STAM-binding protein; AMSH; endosome-associated ubiquitin isopeptidase; associated molecule with the SH3 domain of STAM; MGC126516; MGC126518
• Gene ID: 10617
• mRNA Refseq: NM_006463
• Protein Refseq: NP_006454
• MIM: 606247
• UniProt ID: O95630
• Chromosome Location: 2p24.3-p24.1
• Pathway: Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Internalization of ErbB1, organism-specific biosystem
• Function: metal ion binding; metallopeptidase activity; peptidase activity; protein binding