Active Recombinant Human ATG7, His-tagged

• Cat.No.: ATG7-169H
• Product Overview: Recombinant human ATG7 (amino acid residues 1-706), fused with N-terminal His, was expressed in Insect sf21.

Specification

• Description: Ubiquitin-like proteins (ubl) Autophagy 12 (ATG12) and ATG8 are activated by ATG7 the E1-like activating enzyme. ATG12 and ATG8 are transferred to two E2-like conjugating enzymes ATG10 and ATG3 respectively. Cloning of the human ATG7 gene was first described by Yuan et al. (1999) and ATG7 shares 38% sequence identity to its yeast homologue Apg7. ATG7 can also activate ATG8 mammalian homologues, GABARAP and GATE-16. It forms a homodimer via the C-terminal region that is important for enzyme substrate interaction and E1-E2 complex formation (Komatsu et al. 2005).
• Source: Insect cells
• Species: Human
• Tag: His
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: E1 Thioester GABARAP Loading Assay: The activity of His-ATG7 was validated by loading GABARAP onto the active cysteine of His-ATG7. Incubation of the His-ATG7 enzyme in the presence of GABARAP and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the GABARAP/His-ATG7 thioester bond to the reducing agent DTT was confirmed.
• Molecular Mass: 81.34 kDa
• Purity: >95% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: ATG7 ATG7 autophagy related 7 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ATG7
• Synonyms: ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); APG7 autophagy 7 like (S. cerevisiae) , APG7L; ubiquitin-like modifier-activating enzyme ATG7; DKFZp434N0735; GSA7; hAGP7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ubiquitin activating enzyme E1-like protein; ubiquitin-activating enzyme E1-like protein; APG7L; APG7-LIKE
• Gene ID: 10533
• mRNA Refseq: NM_001136031
• Protein Refseq: NP_001129503
• MIM: 608760
• UniProt ID: O95352
• Chromosome Location: 3p25.3-p25.2
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Regulation of autophagy, organism-specific biosystem
• Function: APG12 activating enzyme activity; catalytic activity; nucleotide binding; protein binding; protein homodimerization activity; ubiquitin activating enzyme activity