Active Recombinant Human YOD1, GST-tagged

• Cat.No.: YOD1-177H
• Product Overview: Recombinant human YOD1 (amino acid residues 1-348), fused with N-terminal His, was expressed in E.coli.

Specification

• Description: OTU enzymes play important roles as negative-feedback regulators in NF-κB signalling, interferon signalling and in p97 (cdc48)-mediated processes although the cellular functions of most OTU enzymes remain to be discovered. Ovarian tumour family DUBs contain a papain-like catalytic core of ~180 amino acids. In addition to their catalytic domain, many OTU members have additional ubiquitin-binding domains (UBDs). At least 20 different UBD families have been described, and knowledge of linkage-specific UBDs have provided the means to understand the roles of different ubiquitin linkages in cells (Licchesi et al., 2012).
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-OTU1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-OTU1 was compared confirming the deubiquitylating activity of GST-OTU1.
• Molecular Mass: ~65 kDa
• Purity: >98% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: YOD1 YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: YOD1
• Synonyms: DUBA8; OTUD2; PRO0907; RP11-164O23.1
• Gene ID: 55432
• mRNA Refseq: NM_018566.3
• Protein Refseq: NP_061036.3
• MIM: 612023
• UniProt ID: Q5VVQ6
• Chromosome Location: 1q32.2
• Pathway: Protein processing in endoplasmic reticulum, organism-specific biosystem;Protein processing in endoplasmic reticulum, conserved biosystem
• Function: protein binding;ubiquitin-specific protease activity;zinc ion binding