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Active Recombinant Human USP9X, GST-tagged

Cat.No. : USP9X-184H
Product Overview : Recombinant human USP9X (amino acid residues 1554-1995), fused with N-terminal GST, was expressed in E.coli.
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Description : USP9X acts by removing monoubiquitin from SMAD4, thereby permitting its association with phospho-SMAD2 and subsequent activation of TGF-β/SMAD-responsive gene targets. USP9X has also been shown to control AMP-activated protein kinase (AMPK)-related kinase activity through direct removal of non-canonical K29 and/or K33-linked ubiquitin chains (Sacco et al., 2010). USP9X can also stabilise levels of myeloid cell leukemia sequence 1 protein (MCL1) and thereby promotes cell survival. USP9X binds MCL1 and removes the Lys 48-linked polyubiquitin chains that normally mark MCL1 for proteasomal degradation.
Source : E. coli
Species : Human
Tag : GST
Form : 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity : Deubiquitylase Enzyme Assay: The activity of GST-USP9x was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-USP9x was compared confirming the deubiquitylating activity of GST-USP9x.
Molecular Mass : ~79 kDa
Purity : >78% by SDS-PAGE
Storage : 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration : 0.5 mg/ml
Gene Name : USP9X ubiquitin specific peptidase 9, X-linked [ Homo sapiens ]
Official Symbol : USP9X
Synonyms : USP9X; ubiquitin specific peptidase 9, X-linked; ubiquitin specific peptidase 9, X linked (fat facets like, Drosophila) , ubiquitin specific protease 9, X chromosome (fat facets like Drosophila) , ubiquitin specific protease 9, X linked (fat facets like, Drosophila); probable ubiquitin carboxyl-terminal hydrolase FAF-X; DFFRX; FAF; hFAM; fat facets in mammals; ubiquitin thioesterase FAF-X; deubiquitinating enzyme FAF-X; ubiquitin thiolesterase FAF-X; fat facets protein related, X-linked; fat facets protein-related, X-linked; Drosophila fat facets related, X-linked; ubiquitin-specific protease 9, X chromosome; ubiquitin-specific processing protease FAF-X; ubiquitin-specific-processing protease FAF-X; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); FAM;
Gene ID : 8239
mRNA Refseq : NM_001039590
Protein Refseq : NP_001034679
MIM : 300072
UniProt ID : Q93008
Chromosome Location : Xp11.4
Function : co-SMAD binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity; peptidase activity; protein binding; ubiquitin thiolesterase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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