Recombinant Human Cystatin B (Stefin B), His-tagged


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Recombinant Human Cystatin B (Stefin B), His-tagged

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Cat. No. : CSTB-299H
Product Overview : Recombinant CSTB, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques (118 aa, 13kDa).
Description : CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus.
Source : E.coli.
Formulation : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl.
Molecular Weight : 13 kDa (118 aa).
Purity : > 95% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Endotoxin Level : < 1.0 EU per 1 μg of protein (determined by LAL method).
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDBrendering based on 1stf.Available structures:1stf, 2oct. :

Gene Name : CSTB
Synonyms : CSTB; cystatin B (stefin B); PME; CST6; EPM1; STFB; CPI-B; Stefin-B; cystatin B; stefin B; cystatin B (stefin B); Liver thiol proteinase inhibitor; cystatin B (liver thiol proteinase inhibitor).
Gene ID : 1476
mRNA Refseq : NM_000100.2
Protein Refseq : NP_000091.1
MIM : 601145
UniProt ID : P04080
Chromosome Location : 21q22.3
Function : cysteine-type endopeptidase inhibitor activity; protease binding; protein binding.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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