Recombinant Human Cystatin B (Stefin B), His-tagged CSTB-299H

Recombinant Human Cystatin B (Stefin B), His-tagged

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Recombinant Human Cystatin B (Stefin B), His-tagged

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Cat. No. : CSTB-299H
Product Overview : Recombinant CSTB, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques (118 aa, 13kDa).
Description : CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus.
Source : E.coli.
Formulation : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl.
Molecular Weight : 13 kDa (118 aa).
Purity : > 95% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Endotoxin Level : < 1.0 EU per 1 μg of protein (determined by LAL method).
Sequence : MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDBrendering based on 1stf.Available structures:1stf, 2oct. :

Gene Name : CSTB
Synonyms : CSTB; cystatin B (stefin B); PME; CST6; EPM1; STFB; CPI-B; Stefin-B; cystatin B; stefin B; cystatin B (stefin B); Liver thiol proteinase inhibitor; cystatin B (liver thiol proteinase inhibitor).
Gene ID : 1476
mRNA Refseq : NM_000100.2
Protein Refseq : NP_000091.1
MIM : 601145
UniProt ID : P04080
Chromosome Location : 21q22.3
Function : cysteine-type endopeptidase inhibitor activity; protease binding; protein binding.

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