Human Daudi lysate


Home / Products / Cell & Tissue Lysates / Cell Line Lysates / Human Daudi lysate

Human Daudi lysate

Daudi Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : Daudi-3H
Species : Human
Concentration : 2 mg/ml
Tissue Type : B Lymphoblast
Preparation method : Tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 g/ml of aprotinin, 5 g/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% -mercaptoethanol.
Recommended Usage : For research use only, not for diagnostic or therapeutic use.
Storage Buffer : Lysate is supplied in SDS sample buffer containing 5% -mercaptoethanol
Applications : Protein Lysate for WB. Western blot-Load 5-20 ug/lane for testing with various antibodies. Heat at 95oC for 2-5 minutes prior to loading on gels.

Products Types

◆ Lysates

Daudi-160HDaudi Whole Cell Lysate+Inquiry

See All Daudi Lysates

Online Inquiry

  • Note: There will be extra charge for optional service!
  • Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2020 Creative BioMart. All Rights Reserved.