Recombinant E.coli DnaK Chaperone Hsp70, Co-chaperone with DnaJ (1-384)
Cat.No. : | DnaK-314E |
Product Overview : | The ATPase domain of recombinant DNAK was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques (384 aa, 41.6 kDa). |
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- Gene Information
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Cat. No. : | DnaK-314 |
Description : | DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK(amino acids1-384) is N-terminal ATPase domain and ATP bound to the ATPase domain induces a conformational change in the substrate binding domain(residues385-638). The protein coding region of the ATPase domain of DNAK (amino acids 1-384) was amplified by PCR and cloned into an E. coli expression vector. |
Source : | E.coli. |
Formulation : | Liquid. In 25 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 5 mM DTT, 10% glycerol. |
Molecular Weight : | 41.6 kDa (384 aa). |
Purity : | > 95% by SDS – PAGE. |
Concentration : | 1 mg/ml (determined by Bradford assay). |
Sequence : | MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTG |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Gene Name : | dnaK |
Synonyms : | dnaK; dnaK chaperone Hsp70, co-chaperone with DnaJ; Hsp70 protein; ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg; chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins. |
Gene ID : | 944750 |
Protein Refseq : | NP_414555.1 |
UniProt ID : | P0A6Y8 |
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Q&As (5)
Ask a questionDNAK interacts with co-chaperones and client proteins, forming complexes that facilitate proper protein folding and function.
Laboratory techniques such as Western blotting and immunohistochemistry are commonly used to analyze DNAK protein expression and localization.
Efforts are underway to develop drugs that modulate DNAK activity, with the potential for treating diseases associated with protein misfolding.
Some studies suggest that DNAK may play a role in cardiovascular health, and its dysregulation could contribute to heart-related disorders.
Monitoring changes in DNAK protein levels during treatment may provide insights into the effectiveness of therapeutic interventions.
Customer Reviews (3)
Write a reviewEqually noteworthy is the remarkable technical support provided by the manufacturer.
Their team of experts has been instrumental in addressing any issues or concerns I have encountered during my experiments.
The DNAK Protein boasts remarkable stability, ensuring its integrity is maintained during storage and experimentation.
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