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Recombinant Mouse Glutathione S-transferase Mu 1, His-tagged

Cat.No. : Gstm1-124M
Product Overview : Recombinant GSTM1 protein was expressed in E.coli and purified by using conventional chromatography techniques, 25.9 kDa (218 aa) (Real molecular weight on SDS-PAGE will be shift up).
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Cat. No. : Gstm1-124M
Description : GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.
Sequences of amino acids : MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK
Formulation : Liquid. In PBS (pH7.4) containing 5 mM glutathione
Purity : > 95% by SDS - PAGE
Concentration : 1 mg/ml (determined by Bradford assay)
Endotoxin Level : < 1.0 EU per 1 μg of protein (determined by LAL method)
Biological activity : Specific activity is 3 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.
Activity Assay : 1. Prepare a 1.mll reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25°C and monitor the A340nm until the value is constant using a spectrophotometer. 3. Add 50ug of recombinant mouse GSTM1 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Record the increase in A340nm for 5 minutes.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : Gstm1 glutathione S-transferase, mu 1 [ Mus musculus ]
Synonyms : EC 2.5.1.18; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; H-B; GST1; MGC26563; MU; OTTHUMP00000013346; OTTHUMP00000013347; GST class-mu 1; HB subunit 4; S-(hydroxyalkyl)glutathione lyase; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; glutathione S-transferase mu 1; Gstb1; Gstb-1; Gstm1; glutathione S-transferase mu 1; GTM1; MU-1; OTTMUSP00000007628; glutathione-S-transferase, mu 1
Gene ID : 14862
mRNA Refseq : NM_010358
Protein Refseq : NP_034488
UniProt ID : P10649
Chromosome Location : 3 F2.3
Pathway : Glutathione metabolism; Metabolism of xenobiotics by cytochrome P450
Function : glutathione transferase activity ; transferase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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