||Recombinant human Rab5a was expressed in E.coli and purified by using conventional chromatography techniques,23 kDa ( 215 aa).
||Rab5a is a member of RAS oncogene family. The small GTPase, Rab5a is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex.
|Sequences of amino acids:
||MASRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE STIGAAFLTQ TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY DITNEESFAR AKNWVKELQR QASPNIVIAL SGNKADLANK RAVDFQEAQS YADDNSLLFM ETSAKTSMNV NEIFMAIAKK LPKNEPQNPG ANSARGRGVD LTEPTQPTRN QCCSN
||Liquid in 20mM Tris-HCl buffer (pH 8.0)
||> 95% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
||< 1.0 EU per 1ug of protein (determined by LAL method)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.