Native Human Complement C4b

• Cat.No.: Complement C4b-51H
• Product Overview: Native Human Complement C4b was purified from Normal human serum (shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-1 and HIV-II).

Specification

• Species: Human
• Source: Normal human serum
• Description: Native human C4b is a glycosylated (~7%) polypeptide containing three disulfide-linked chains. The molecular weight predicted from the amino acid sequence is 180,500 and that observed is 195 kDa. On reduced SDS-PAGE gels C4b shows a three chain structure with an alpha prime chain (α'-chain) of 87 kDa, a beta chain of 75,000 Da and a gamma chain of 33,000 Da. Alpha prime and beta chains are linked through a single disulfide bond. The pI of C4b is approx. 7.5. Cleavage of surface-bound C4b by factor I yields the soluble C4c fragment (147 kDa) and the cell-bound C4d fragment (45 kDa). Upon cleavage of C4 (205 kDa) by C1s, C4a (77 amino acid fragment, 8,759 Da) is released from the N-terminal of the alpha chain and C4b (195 kDa) becomes attached covalently to the surface of the activator.
• Form: Liquid
• Extinction Coeff.: A280 nm = 1.03 at 1.0 mg/mL
• Purity: >90% by SDS-PAGE
• Note: Use normal precautions for handling human blood products.
• Storage: At -70 centigrade or below. Avoid freeze/thaw.
• Storage Buffer: 10 mM Sodium phosphate, 145 mM NaCl, pH 7.3
• Concentration: 1.0 mg/mL

Gene Information

• Official Symbol: Complement C4b
• Synonyms: Complement C4b