||Recombinant SULT1E1, fused to His-tag at C-terminus, was expressed in E.coli and was purified by conventional chromatography, 36.1kDa (302aa).
||Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. Estrogen sulfotransferase may control the level of the estrogen receptor by sulfurylating free estradiol.
|Sequences of amino acids:
||MNSELDYYEK FEEVHGILMY KDFVKYWDNV EAFQARPDDL VIATYPKSGT TWVSEIVYMI YKEGDVEKCK EDVIFNRIPF LECRKENLMN GVKQLDEMNS PRIVKTHLPP ELLPASFWEK DCKIIYLCRN AKDVAVSFYY FFLMVAGHPN PGSLPEFVEK FMQGQVPYGS WYKHVKSWWE KGKSPRVLFL FYEDLKEDIR KEVIKLIHFL ERKPSEELVD RIIHHTSFQE MKNNPSTNYT TLPDEIMNQK LSPFMRKGIT GDWKNHFTVA LNEKFDKHYE QQMKESTLKF RTEILEHHHH HH
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol.
||> 95% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
||< 1.0 EU per 1 μg of protein (determined by LAL method)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.