Recombinant Human Thioredoxin2
Cat.No. : | TXN2-133H |
Product Overview : | Recombinant Thioredoxin2 was expressed in E.coli and purified by using conventional chromatography techniques, 11 kDa (108 aa). |
- Specification
- Gene Information
- Related Products
Cat. No. : | TXN2-133H |
Description : | Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The Thioredoxin2 may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. |
Sequences of amino acids : | MTTFNIQDGP DFQDRVVNSE TPVVVDFHAQ WCGPCKILGP RLEKMVAKQH GKVVMAKVDI DDHTDLAIEY EVSAVPTVLA MKNGDVVDKF VGIKDEDQLE AFLKKLIG |
Formulation : | Liquid. In PBS (pH 7.4) |
Purity : | > 95% by SDS - PAGE |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Biological activity : | Specific activity is 3-4 A650/min/mg and was obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin. |
Activity Assay : | 1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant thioredoxin2 into reaction cocktail and mix immediately. 3. Incubate at 25C for 20 minutes. 4. Record the increase in A650nm for 15 minutes. |
Storagee : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
GeneIDD : | 25828 |
Gene Name : | |
Synonyms : | MT-TRX; MTRX; Mt-Trx; TRX2; Thioredoxin-2; mitochondrial thioredoxin ; thioredoxin 2; TXN2 |
mRNA Refseq : | NM_012473 |
Protein Refseq : | NP_036605 |
MIM : | 609063 |
UniProt ID : | Q99757 |
Chromosome Location : | 22q13.1 |
Function : | electron carrier activity; protein disulfide oxidoreductase activity |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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