"TXN2" Related Products

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Recombinant Human Thioredoxin2

Cat. No.: TXN2-133H
Product Overview: Recombinant Thioredoxin2 was expressed in E.coli and purified by using conventional chromatography techniques, 11 kDa (108 aa).
Description: Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The Thioredoxin2 may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis.
Sequences of amino acids: MTTFNIQDGP DFQDRVVNSE TPVVVDFHAQ WCGPCKILGP RLEKMVAKQH GKVVMAKVDI DDHTDLAIEY EVSAVPTVLA MKNGDVVDKF VGIKDEDQLE AFLKKLIG
Formulation: Liquid. In PBS (pH 7.4)
Purity: > 95% by SDS - PAGE
Concentration: 1 mg/ml (determined by Bradford assay)
Biological activity: Specific activity is 3-4 A650/min/mg and was obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Activity Assay: 1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant thioredoxin2 into reaction cocktail and mix immediately. 3. Incubate at 25C for 20 minutes. 4. Record the increase in A650nm for 15 minutes.
Storagee: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
GeneIDD: 25828
Gene Name:
Synonyms: MT-TRX; MTRX; Mt-Trx; TRX2; Thioredoxin-2; mitochondrial thioredoxin ; thioredoxin 2; TXN2
mRNA Refseq: NM_012473
Protein Refseq: NP_036605
MIM: 609063
UniProt ID: Q99757
Chromosome Location: 22q13.1
Function: electron carrier activity; protein disulfide oxidoreductase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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