||Recombinant human Synaptobrevin1, fused to His- tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (111 aa, 11.9 kDa).
||Synaptobrevin 1(Vehicle-associated membrane, VAMP1) is one of the key proteins in the SNARE complex which is involved in regulated exocytosis. Synaptobrevin1 binds to t-SNAREs, syntaxin (STX) and SNAP25, after the fusion of synaptic vesicles to plasma membrane.
||Liquid. In PBS (pH 7.4) containing 1 mM DTT.
||> 95% by SDS-PAGE.
||1 mg/ml (determined by Bradford assay).
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MSAPAQPPAE GTEGTAPGGGPPGPPPNMT S NRRLQQTQAQ VEEVVDIIRV NVDKVLERDQ KLSELDDRAD ALQAGASQF E SSAAKLKRKY W
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.