Recombinant E.coli mutM


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Recombinant E.coli mutM

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Cat.No. : mutM-52E
Product Overview : A recombinant E. coli strain carrying the cloned fpg gene.
Description : Fpg (also known as Formamidopyrimidine DNA glycosylase, Mut M, FAPY DNA Glycosylase, and 8-oxoguanine DNA glycosylase) participates in the base-excision (BER) pathway of DNA repair enzymes and acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic/apyrimidinic (AP site). The AP-lyase activity cleaves both the 3′ and 5′ phosphodiester bonds at the AP site, producing a 1 base gap in the DNA and 3′ and 5′ phosphate termini. Bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.
Source : E. coli
Species : E. coli
Bio-activity : 20,513 U/mg
Molecular Mass : 30,290 Daltons
Purity : >99% by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34mer oligo-nucleotide duplex containing an 8-oxoguanine base paired with a cysteine in 1 hour at 37°C in a total reaction volume of 10μl in reaction buffer.
Storage : -25 to -15°C
Concentration : 8,000 U/ml
Storage Buffer : Supplied in: 20mM Tris-HCL, 50 mM KCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 8.0 @ 25?C. Supplied with: 10X Yellow Buffer: 100mM Bis-Tris Propane, 100mM MgCl2, 10mM DTT pH 7.0 @ 25?C.
Gene Name : mutM?formamidopyrimidine/5-formyluracil/ 5-hydroxymethyluracil DNA glycosylase [?Escherichia coli str. K-12 substr. MG1655?]
Official Symbol : mutM
Synonyms : mutM; formamidopyrimidine/5-formyluracil/ 5-hydroxymethyluracil DNA glycosylase; ECK3625; fpg; JW3610; NP_418092.1; EC; formamidopyrimidine DNA glycosylase
Gene ID : 946765
Protein Refseq : NP_418092
Pathway : Base excision repair
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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