Active Recombinant E.coli mutM

• Cat.No.: mutM-52E
• Product Overview: A recombinant E. coli strain carrying the cloned fpg gene.

Specification

• Description: Fpg (also known as Formamidopyrimidine DNA glycosylase, Mut M, FAPY DNA Glycosylase, and 8-oxoguanine DNA glycosylase) participates in the base-excision (BER) pathway of DNA repair enzymes and acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic/apyrimidinic (AP site). The AP-lyase activity cleaves both the 3′ and 5′ phosphodiester bonds at the AP site, producing a 1 base gap in the DNA and 3′ and 5′ phosphate termini. Bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.
• Source: E. coli
• Species: E. coli
• Bio-activity: 20,513 U/mg
• Molecular Mass: 30,290 Daltons
• Purity: >99% by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34mer oligo-nucleotide duplex containing an 8-oxoguanine base paired with a cysteine in 1 hour at 37°C in a total reaction volume of 10μl in reaction buffer.
• Storage: -25 to -15°C
• Concentration: 8,000 U/ml
• Storage Buffer: Supplied in: 20mM Tris-HCL, 50 mM KCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 8.0 @ 25?C. Supplied with: 10X Yellow Buffer: 100mM Bis-Tris Propane, 100mM MgCl2, 10mM DTT pH 7.0 @ 25?C.

Gene Information

• Gene Name: mutM?formamidopyrimidine/5-formyluracil/ 5-hydroxymethyluracil DNA glycosylase [?Escherichia coli str. K-12 substr. MG1655?]
• Official Symbol: mutM
• Synonyms: mutM; formamidopyrimidine/5-formyluracil/ 5-hydroxymethyluracil DNA glycosylase; ECK3625; fpg; JW3610; NP_418092.1; EC 3.2.2.23; formamidopyrimidine DNA glycosylase
• Gene ID: 946765
• Protein Refseq: NP_418092
• Pathway: Base excision repair