Recombinant E.coli nei nei-53E

Recombinant E.coli nei


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Recombinant E.coli nei

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Cat.No. : nei-53E
Product Overview : An E. coli strain which carries the cloned Endonuclease VIII gene.
Description : E.coli Endonuclease VIII functions as both an N-glycosylase (by excising oxidative base lesions) and an AP lyase (by subsequently cleaving the phosphodiester backbone), leaving terminal phosphates at the 5′ and 3′ ends. Damaged bases removed by Endonuclease VIII include: urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydanton, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methyltartronylurea.
Source : E. coli
Species : E. coli
Bio-activity : 770,000 U/mg
Molecular Mass : 29,845 Daltons
Purity : >99% by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to cleave 1 pmol of an oligonucleotide duplex containing a single AP site in 1 hour at 37°C.
Storage : -25 to -15°C
Concentration : 10,000 U/mL
Storage Buffer : Supplied in: 10mM Tris-HCL,250 mM NaCl, 0.1mM EDTA, 50% glycerol pH 8.0 @ 25?C. Supplied with: 10X Endonuclease Vlll Buffer: 100mM Tris-HCl, 750mM NaCl, 10mM EDTA pH 8.0 @ 25?C.
Gene Name : nei endonuclease VIII [ Escherichia coli str. K-12 substr. W3110 ]
Official Symbol : nei
Synonyms : nei; endonuclease VIII; YP_488994.1; 5-formyluracil/5-hydroxymethyluracil DNA glycosylase~ECK0703:JW0704:b0714
Gene ID : 12930934
Protein Refseq : YP_488994
UniProt ID : AP_001352
Pathway : Base excision repair

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