Active Recombinant E.coli Exonuclease I

• Cat.No.: Exonuclease I-54E
• Product Overview: Purified from a strain of?E. coli?that expresses the recombinant Exonuclease I gene.

Specification

• Description: Exonuclease I cleaves single-stranded DNA in the 3′→5′ direction, releasing 5′ -mono/di-nucleotides and leaving double-stranded DNA molecules and the 5′-terminus intact. The enzyme is processive though digestion is inhibited by the presence of a 3′ -terminal phosphate. Exonuclease I is tolerant of a wide-range of buffer conditions and can typically be added to reactions containing magnesium.
• Source: E. coli
• Species: E. coli
• Bio-activity: 185,000 U/mg
• Molecular Mass: 54,500 Daltons
• Purity: >99% by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble total nucleotide in 30 minutes at 37°C.
• Storage: -15 to -25°C
• Concentration: 20,000 U/ml
• Storage Buffer: Supplied in: 10mM Tris-HCl, 100mM NaCl, 1mM DTT, 0.5mM EDTA, 50% glycerol pH 7.5 @ 25?C.