Recombinant E.coli Exonuclease I Exonuclease I-54E

Recombinant E.coli Exonuclease I


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Recombinant E.coli Exonuclease I

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Cat.No. : Exonuclease I-54E
Product Overview : Purified from a strain of?E. coli?that expresses the recombinant Exonuclease I gene.
Description : Exonuclease I cleaves single-stranded DNA in the 3′→5′ direction, releasing 5′ -mono/di-nucleotides and leaving double-stranded DNA molecules and the 5′-terminus intact. The enzyme is processive though digestion is inhibited by the presence of a 3′ -terminal phosphate. Exonuclease I is tolerant of a wide-range of buffer conditions and can typically be added to reactions containing magnesium.
Source : E. coli
Species : E. coli
Bio-activity : 185,000 U/mg
Molecular Mass : 54,500 Daltons
Purity : >99% by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble total nucleotide in 30 minutes at 37°C.
Storage : -15 to -25°C
Concentration : 20,000 U/ml
Storage Buffer : Supplied in: 10mM Tris-HCl, 100mM NaCl, 1mM DTT, 0.5mM EDTA, 50% glycerol pH 7.5 @ 25?C.

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Exonuclease I

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