"pseT" Related Products

Recombinant Bacteriophage T4 Polynucleotide Kinase

Cat.No.: pseT-58B
Product Overview: Purified from a strain of?E.coli?that expresses the recombinant T4 Polynucleotide Kinase gene.
Description: T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules. T4 PNK also exhibits 3′-phosphatase and 2′, 3′ cyclicphosphodiesterase activities.
Source: E. coli
Species: Bacteriophage T4
Bio-activity: 133,333 U/mg
Molecular Mass: 34,620 Daltons
Purity: >99% by SDS-PAGE
Unit Definition: 1 unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of [32P] (ATP donor) in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer.
Storage: -25 to -15°C
Concentration: 10,000 U/m
Storage Buffer: Supplied in: 10mM Tris-HCL, 50 mM KCl, 0.1 μM ATP, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.4 @ 25?C. Supplied with: 10X T4 Polynucleotide Kinase Buffer: 700mM Tris-HCl, 100mM MgCl2, 50mM DTT pH 7.6 @ 25?C.
Gene Name: pseT?polynucleotide kinase [?Enterobacteria phage T4?]
Official Symbol: pseT
Synonyms: pseT; polynucleotide kinase; NP_049834.1; 5"-hydroxyl kinase, a 3"-phosphatase and a 2",3"-cyclic phosphodiesterase; catalyzes the transfer of the terminal phosphate of ATP to the 5"-hydroxyl termini of ribo- and deoxyribonucleotides; in the presence of ADP the enzyme also catalyzes the exchange of the 5" terminal phosphate from phosphorylated DNA to ADP; these activities modify the ends of nicked tRNA generated by a bacterial response to infection and facilitate repair by T4 RNA ligase
Gene ID: 1258796
Protein Refseq: NP_049834
UniProt ID: 2IA5_D

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.