| Cat.No.: | pseT-58B |
| Product Overview: | Purified from a strain of?E.coli?that expresses the recombinant T4 Polynucleotide Kinase gene. |
| Description: | T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules. T4 PNK also exhibits 3′-phosphatase and 2′, 3′ cyclicphosphodiesterase activities. |
| Source: | E. coli |
| Species: | Bacteriophage T4 |
| Bio-activity: | 133,333 U/mg |
| Molecular Mass: | 34,620 Daltons |
| Purity: | >99% by SDS-PAGE |
| Unit Definition: | 1 unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of [32P] (ATP donor) in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer. |
| Storage: | -25 to -15°C |
| Concentration: | 10,000 U/m |
| Storage Buffer: | Supplied in: 10mM Tris-HCL, 50 mM KCl, 0.1 μM ATP, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.4 @ 25?C. Supplied with: 10X T4 Polynucleotide Kinase Buffer: 700mM Tris-HCl, 100mM MgCl2, 50mM DTT pH 7.6 @ 25?C. |
| Gene Name: | pseT?polynucleotide kinase [?Enterobacteria phage T4?] |
| Official Symbol: | pseT |
| Synonyms: | pseT; polynucleotide kinase; NP_049834.1; 5"-hydroxyl kinase, a 3"-phosphatase and a 2",3"-cyclic phosphodiesterase; catalyzes the transfer of the terminal phosphate of ATP to the 5"-hydroxyl termini of ribo- and deoxyribonucleotides; in the presence of ADP the enzyme also catalyzes the exchange of the 5" terminal phosphate from phosphorylated DNA to ADP; these activities modify the ends of nicked tRNA generated by a bacterial response to infection and facilitate repair by T4 RNA ligase |
| Gene ID: | 1258796 |
| Protein Refseq: | NP_049834 |
| UniProt ID: | 2IA5_D |
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