Recombinant Bacteriophage T4 Polynucleotide Kinase
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| Cat.No. : | pseT-58B |
| Product Overview : | Purified from a strain of?E.coli?that expresses the recombinant T4 Polynucleotide Kinase gene. |
| Description : | T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules. T4 PNK also exhibits 3′-phosphatase and 2′, 3′ cyclicphosphodiesterase activities. |
| Source : | E. coli |
| Species : | Bacteriophage T4 |
| Bio-activity : | 133,333 U/mg |
| Molecular Mass : | 34,620 Daltons |
| Purity : | >99% by SDS-PAGE |
| Unit Definition : | 1 unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of [32P] (ATP donor) in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer. |
| Storage : | -25 to -15°C |
| Concentration : | 10,000 U/m |
| Storage Buffer : | Supplied in: 10mM Tris-HCL, 50 mM KCl, 0.1 μM ATP, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.4 @ 25?C. Supplied with: 10X T4 Polynucleotide Kinase Buffer: 700mM Tris-HCl, 100mM MgCl2, 50mM DTT pH 7.6 @ 25?C. |
| Gene Name : | pseT?polynucleotide kinase [?Enterobacteria phage T4?] |
| Official Symbol : | pseT |
| Synonyms : | pseT; polynucleotide kinase; NP_049834.1; 5"-hydroxyl kinase, a 3"-phosphatase and a 2",3"-cyclic phosphodiesterase; catalyzes the transfer of the terminal phosphate of ATP to the 5"-hydroxyl termini of ribo- and deoxyribonucleotides; in the presence of ADP the enzyme also catalyzes the exchange of the 5" terminal phosphate from phosphorylated DNA to ADP; these activities modify the ends of nicked tRNA generated by a bacterial response to infection and facilitate repair by T4 RNA ligase |
| Gene ID : | 1258796 |
| Protein Refseq : | NP_049834 |
| UniProt ID : | 2IA5_D |
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