Recombinant Bacteriophage T4 Polynucleotide Kinase


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Recombinant Bacteriophage T4 Polynucleotide Kinase

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Cat.No. : pseT-58B
Product Overview : Purified from a strain of?E.coli?that expresses the recombinant T4 Polynucleotide Kinase gene.
Description : T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules. T4 PNK also exhibits 3′-phosphatase and 2′, 3′ cyclicphosphodiesterase activities.
Source : E. coli
Species : Bacteriophage T4
Bio-activity : 133,333 U/mg
Molecular Mass : 34,620 Daltons
Purity : >99% by SDS-PAGE
Unit Definition : 1 unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of [32P] (ATP donor) in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer.
Storage : -25 to -15°C
Concentration : 10,000 U/m
Storage Buffer : Supplied in: 10mM Tris-HCL, 50 mM KCl, 0.1 μM ATP, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.4 @ 25?C. Supplied with: 10X T4 Polynucleotide Kinase Buffer: 700mM Tris-HCl, 100mM MgCl2, 50mM DTT pH 7.6 @ 25?C.
Gene Name : pseT?polynucleotide kinase [?Enterobacteria phage T4?]
Official Symbol : pseT
Synonyms : pseT; polynucleotide kinase; NP_049834.1; 5"-hydroxyl kinase, a 3"-phosphatase and a 2",3"-cyclic phosphodiesterase; catalyzes the transfer of the terminal phosphate of ATP to the 5"-hydroxyl termini of ribo- and deoxyribonucleotides; in the presence of ADP the enzyme also catalyzes the exchange of the 5" terminal phosphate from phosphorylated DNA to ADP; these activities modify the ends of nicked tRNA generated by a bacterial response to infection and facilitate repair by T4 RNA ligase
Gene ID : 1258796
Protein Refseq : NP_049834
UniProt ID : 2IA5_D
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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