Active Recombinant E. coli Uracil DNA Glycosylase

• Cat.No.: ung-61E
• Product Overview: A recombinant?E. coli?strain carrying the Uracil DNA Glycosylase gene from?E. coli?K-12.

Specification

• Description: Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or rna>
• Source: E. coli
• Species: E. coli
• Bio-activity: 77,000 U/mg
• Molecular Mass: 25,693 Daltons
• Purity: >99% by SDS-PAGE
• Unit Definition: 1 unit is defined as the amount of enzyme that catalyzes the release of 1.8 nmol of Uracil in 30 minutes from double-stranded, tritiated, Uracil containing-DNA at 37°C in 1X UDG Reaction Buffer.
• Storage: -15 to -25°C
• Concentration: 2,000 U/ml
• Storage Buffer: Supplied in: 10mM Tris-HCl, 50mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.5 @ 25?C.Supplied with: 10X UDG Reaction Buffer: 200mM Tris-HCl, 10mM DTT, 10mM EDTA pH 8.0 @ 25?C.

Gene Information

• Gene Name: ung uracil-DNA-glycosylase [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: ung
• Synonyms: ung; uracil-DNA-glycosylase; ECK2578; JW2564; NP_417075.1; EC 3.2.2.-
• Gene ID: 947067
• Protein Refseq: NP_417075
• Pathway: Base excision repair,