Recombinant E. coli RNase H


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Recombinant E. coli RNase H

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Cat.No. : rnh-63E
Product Overview : A recombinant?E. coli?strain carrying the RNAse H (rnh) gene from?E.coli.
Description : E.coli?RNase H (rnh) is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5-phosphate and 3-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.
Source : E. coli
Species : E. coli
Bio-activity : 625,000 U/mg
Purity : >99% by SDS-PAGE
Unit Definition : 1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.
Storage : -25 to -15°C
Concentration : 5,000 U/ml
Storage Buffer : Supplied in: 20 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol, pH 7.9 @ 25°C Supplied with: 10X RNAse H Buffer: 500mM Tris-HCl, 750 mM KCl, 30 mM MgCl2, 100 mM DTT, pH 8.3 @ 25?C.

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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