Recombinant Human PON1, His-tagged

• Cat.No.: PON1-07H
• Product Overview: Recombinant Human PON1(Gln35~Val206), fused with N-terminal His-Tag, was expressed in E.coli.

Specification

• Description: The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Molecular Mass: 20.8 kDa
• Protein length: Gln35~Val206
• AA Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.MGHHHHHHSGSEF-QPVELP NCNLVKGIET GSEDLEILPN GLAFISSGLK YPGIKSFNPN SPGKILLMDL NEEDPTVLEL GITGSKFDVS SFNPHGISTF TDEDNAMYLL VVNHPDAKST VELFKFQEEE KSLLHLKTIR HKLLPNLNDI VAVGPEHFYG TNDHYFLDPY LQSWEMYLGL AWSYVV
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37ºC for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PON1 paraoxonase 1 [ Homo sapiens (human) ]
• Official Symbol: PON1
• Synonyms: PON1; paraoxonase 1; PON; serum paraoxonase/arylesterase 1; ESA; esterase A; K-45; PON 1; A-esterase 1; paraoxonase B-type; aromatic esterase 1; arylesterase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1; MVCD5
• Gene ID: 5444
• mRNA Refseq: NM_000446
• Protein Refseq: NP_000437
• MIM: 168820
• UniProt ID: P27169
• Chromosome Location: 7q21.3
• Pathway: Metabolic pathways; Phase I, non P450
• Function: aryldialkylphosphatase activity; arylesterase activity; calcium ion binding