Recombinant Human PON1, His-tagged

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Recombinant Human PON1, His-tagged

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Cat.No. : PON1-07H
Product Overview : Recombinant Human PON1(Gln35~Val206), fused with N-terminal His-Tag, was expressed in E.coli.
Description : The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3.
Source : E.coli
Species : Human
Tag : His
Form : Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Molecular Mass : 20.8 kDa
Protein length : Gln35~Val206
AA Sequence : The target protein is fused with N-terminal His-Tag, its sequence is listed below.MGHHHHHHSGSEF-QPVELP NCNLVKGIET GSEDLEILPN GLAFISSGLK YPGIKSFNPN SPGKILLMDL NEEDPTVLEL GITGSKFDVS SFNPHGISTF TDEDNAMYLL VVNHPDAKST VELFKFQEEE KSLLHLKTIR HKLLPNLNDI VAVGPEHFYG TNDHYFLDPY LQSWEMYLGL AWSYVV
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37ºC for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name : PON1 paraoxonase 1 [ Homo sapiens (human) ]
Official Symbol : PON1
Synonyms : PON1; paraoxonase 1; PON; serum paraoxonase/arylesterase 1; ESA; esterase A; K-45; PON 1; A-esterase 1; paraoxonase B-type; aromatic esterase 1; arylesterase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1; MVCD5
Gene ID : 5444
mRNA Refseq : NM_000446
Protein Refseq : NP_000437
MIM : 168820
UniProt ID : P27169
Chromosome Location : 7q21.3
Pathway : Metabolic pathways; Phase I, non P450
Function : aryldialkylphosphatase activity; arylesterase activity; calcium ion binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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