Recombinant Human PON3, His-tagged PON3-27H

Recombinant Human PON3, His-tagged


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Recombinant Human PON3, His-tagged

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Cat.No. : PON3-27H
Product Overview : A DNA sequence encoding the human PON3 (Q15166-1) (Met 1-Leu 354) (50 Ser/Asn) was expressed, with a polyhistidine tag at the N-terminus.
Description : This gene is a member of the paraoxonase family and lies in a cluster on chromosome 7 with the other two family members. The encoded protein is secreted into the bloodstream and associates with high-density lipoprotein (HDL). The protein also rapidly hydrolyzes lactones and can inhibit the oxidation of low-density lipoprotein (LDL), a function that is believed to slow the initiation and progression of atherosclerosis. Alternatively spliced variants which encode different protein isoforms have been described; however, only one has been fully characterized.
Source : Baculovirus-Insect Cells
Species : Human
Tag : His
Predicted N Terminal : Met
Form : Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 1mM CaCL2, 10% gly, 0.1% DDM. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Molecular Mass : The recombinant human PON3 consists of 373 amino acids and predicts a molecular mass of 42 kDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 85 % as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -70?
Storage : Store it under sterile conditions at -20? to -80?. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : A hardcopy of COA with reconstitution instruction is sent along with the products.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : PON3 paraoxonase 3 [ Homo sapiens (human) ]
Official Symbol : PON3
Synonyms : PON3; paraoxonase 3; serum paraoxonase/lactonase 3; paraoxanase-3; arylesterase 3; NP_000931.1; EC; EC; EC
Gene ID : 5446
mRNA Refseq : NM_000940
Protein Refseq : NP_000931
MIM : 602720
UniProt ID : Q15166
Chromosome Location : 7q21.3
Pathway : Metabolic pathways; Phase I, non P450
Function : 3,4-dihydrocoumarin hydrolase activity; aryldialkylphosphatase activity; arylesterase activity

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