"KBP" Related Products

FabAffinity KBP Agarose High Flow

Cat.no: FKBP-002A
Product Overview: Recombinant human Kappa Binding Protein is also named as KBP. FabAffinity KBP Agarose High Flow Resin, with KBP coupled to high flow Agarose matrix, is a new choice for purification of human antibodies and antibody fragments, which contain the constant domain of the Kappa light chain.
Form: Slurry in 20% Ethanol
Size: 25 mL
Matrix: Highly cross-linked Agarose
Molecular Characterization: KBP is fused with a 6×his tag at C-terminus, and has a calculated molecular mass of 16 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of KBP is approximately 16 kDa.
Average particle size: 90 μm
Ligand: kappa binding protein (HEK derived)
Coupling chemistry: Epoxy
Dynamic binding capacity: 10mg Fab /ml media
Recommended flow rate: 100-300cm/h
Viscosity: Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened and keep container tightly closed in a dry and well-entilated place.Packed columns should be equilibrated in binding buffer containing 20% ethanol to prevent microbial growth.After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
Recommended column height: 5‐20cm
pH working range: 3-10
pH CIP range: 2-11
CIP stability: 10mM NaOH, 6M Gua-HCl, 0.1M Acetate acid and 30% IPA
Temperature Stability: 2‐40 °C
Shelf life: 5 years
Products Types ◆ Recombinant Protein

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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