"NHS" Related Products

NHS Activated Agarose High Flow

Cat.no: NHS-001A
Product Overview: NHS Actived Agarose High Flow is a recently developed pre-activated agarose matrix that increases the choice of coupling chemistries available to these users. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. NHS Actived Agarose High Flow provides a spacer arm and is therefore particularly suitable for immobilizing small protein and peptide ligands.
Size: 25 mL, 500 mL, 5 L
Matrix: Highly cross-linked 4% agarose, spherical
Functional group: N-hydroxysuccinimide (NHS)
Average particle size: 90 μm
Ligand: N-hydroxysuccinimide (NHS) functional groups
Dynamic binding capacity: >30mg/mL (slurry) or >25mg/mL (dry resin)
Recommended flow rate: 150cm/h at 100 kPa
Viscosity: Before storage we recommend to wash the column volumns with of 20% ethanol to prevent microbial growth. Store the column in 20% ethanol at +4-8°C.
Recommended column height: 5‐20cm
pH working range: 3-13
pH CIP range: 2-13
Temperature Stability: 2‐40 °C
Elution buffer: 0.1 M glycine-HCl, pH 2.7

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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