CM Sepharose Agarose

• Cat.No.: carboxy methyl-003C
• Product Overview: CM Sepharose Agarose is a weak cation exchanger based on the well established Sepharose ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Specification

• Description: CM Sepharose Agarose is composed of cross-linked, 6% agarose particles, with carboxymethyl (CM) weak cation exchange groups. It has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.
• Storage: Store at 4 to 30 centigrade, 20% Ethanol
• Size: 25 mL, 500 mL, 10 L, 60 L
• Matrix: Cross-linked agarose, 6%, spherical
• Ionic Exchanger Type: Weak cation exchanger
• Ion exchange capacity: 0.09-0.13 mmol H+/ml medium
• MW exclusion limit (kD): Globular Proteins： ~4000
• Average particle size: 45-165 μm
• Ligand: Carboxymethyl
• Chemical stability: Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
• Physical stability: Pressure/Flow Specification: 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
• pH working range: 4–13 (pH range where resin can be operated without significant change in function)
  pH ligand fully charged(pH range where ligand is fully charged): Above 6
• pH CIP range: 2–14 (pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function)