CM Sepharose Agarose


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CM Sepharose Agarose

Carboxymethyl Related Products

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Cat.No. : carboxy methyl-003C
Product Overview : CM Sepharose Agarose is a weak cation exchanger based on the well established Sepharose ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
Description : CM Sepharose Agarose is composed of cross-linked, 6% agarose particles, with carboxymethyl (CM) weak cation exchange groups. It has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.
Storage : Store at 4 to 30 centigrade, 20% Ethanol
Size : 25 mL, 500 mL, 10 L, 60 L
Matrix : Cross-linked agarose, 6%, spherical
Ionic Exchanger Type : Weak cation exchanger
Ion exchange capacity : 0.09-0.13 mmol H+/ml medium
MW exclusion limit (kD) : Globular Proteins: ~4000
Average particle size : 45-165 μm
Ligand : Carboxymethyl
Chemical stability : Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Physical stability : Pressure/Flow Specification: 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
pH working range : 4–13 (pH range where resin can be operated without significant change in function)
pH ligand fully charged(pH range where ligand is fully charged): Above 6
pH CIP range : 2–14 (pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function)

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