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| Cat.no : | Methylsulphonate-003C |
| Product Overview : | S strong ion exchangers are highly cross-linked, surface modified media with high dynamic binding capacities and stable at high flow velocities. These high performances of the optimized media offer significant opportunity for increasing downstream purification throughput. |
| Matrix : | Highly cross-linked cellulose with dextran scaffold |
| Ion exchange capacity : | 0.13-0.25 meq /ml |
| Functional group : | Methylsulphonate: -CH2SO3– |
| Protein : | Methylsulphonate |
| Average particle size : | 40 – 130 um |
| Dynamic binding capacity : | >130mg IgG/ml gel |
| Recommended flow rate : | 1,000 cm/h in a 2.2cm diameter column with 20cm bed height at<>Over 500cm/h 30cm diameter column with 20cm bed height at<> |
| Viscosity : | Store unopened container at ambient temperature. Do not freeze.Short-term storage for bulk and column (2 weeks or less) can be at a room temperature with pH 2 to pH 14. It is possible to store under alkaline condition at less than 20°C as recommendation.0.1M NaOH ; 2-4 months0.5M NaOH ; less than 1 monthLonger storage should be in neutral buffer containing 20 % ethanol, at 2 – 8 °C. Do not freeze. |
| Chemical stability : | Stable in:Most salts (NaCl, (NH4)2SO4, etc.), Alcohol ( 30 % (v/v) IPA, 70 % (v/v) EtOH), Urea (6M) and Guanidine-HCl (6M); pH 2 to pH14 (MAX S-r) , pH3 to pH14 (MAX S-h) at 40oC,1 week 0.1M NaOH at less than 20oC, 3 months (MAX S-r & S-h) |
| Physical stability : | Stable in:Most salts (NaCl, (NH4)2SO4, etc.), Alcohol ( 30 % (v/v) IPA, 70 % (v/v) EtOH), Urea (6M) and Guanidine-HCl (6M); pH 2 to pH14 (MAX S-r) , pH3 to pH14 (MAX S-h) at 40oC,1 week 0.1M NaOH at less than 20oC, 3 months (MAX S-r & S-h) |
| pH working range : | 2 – 13 |
| pH CIP range : | 3 - 14 |
| Elution buffer : | 0.1 – 2.0 M sodium chloride in adsorption buffer |
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