Active Recombinant Human EPHA7, His & GST-tagged

• Cat.No.: EPHA7-7326H
• Product Overview: Recombinant Human EPHA7 (NP_004431) (Gly579-Val998), fused with the N-terminal polyhistidine-tagged GST tag, was epreesed in Baculovirus-Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 579-998 a.a.
• Predicted N Terminal: Met
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Bio-activity: The specific activity was determined to be 9.5 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate (see protocol for kinase assay).
• Molecular Mass: The recombinant human EPHA7 /GST chimera consists of 657 amino acids and has a calculated molecular mass of 75.2 kDa. The recombinant protein migrates as an approximately 76 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 94 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC
• Storage: Store it under sterile conditions at -20ºC to -80ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Shipping: Liquid. It is shipped out with blue ice.

Gene Information

• Gene Name: EPHA7 EPH receptor A7 [ Homo sapiens (human) ]
• Official Symbol: EPHA7
• Synonyms: EPHA7; EPH receptor A7; EphA7; ephrin type-A receptor 7; Hek11; EK11; EHK-3; EPH-like kinase 11; EPH homology kinase 3; Eph homology kinase-3; receptor protein-tyrosine kinase HEK11; tyrosine-protein kinase receptor EHK-3; EHK3; HEK11
• Gene ID: 2045
• mRNA Refseq: NM_004440
• Protein Refseq: NP_004431
• MIM: 602190
• UniProt ID: Q15375
• Chromosome Location: 6q16.1
• Pathway: Axon guidance; Developmental Biology; EPH-ephrin mediated repulsion of cells; EPHA forward signaling
• Function: ATP binding; GPI-linked ephrin receptor activity; axon guidance receptor activity