||Recombinant Human MDM2 was expressed in E. coli.
||This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
||50 mM Hepes pH 8.0, 200 mM NaCl, 10% glycerol, 5 mM TCEP
||Recombinant Human Mdm2/Hdm2 is a RING finger Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Mdm2/Hdm2 concentration of 0.5-5 µM.
||>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
||Avoid repeated freeze-thaw cycles.12 months from date of receipt, -70 ºC as supplied.3 months, -70 ºC under sterile conditions after opening.