Active Recombinant Human MDM2

• Cat.No.: MDM2-2319H
• Product Overview: Recombinant Human MDM2 was expressed in E. coli.

Specification

• Description: This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
• Source: E. coli
• Species: Human
• Form: 50 mM Hepes pH 8.0, 200 mM NaCl, 10% glycerol, 5 mM TCEP
• Bio-activity: Recombinant Human Mdm2/Hdm2 is a RING finger Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Mdm2/Hdm2 concentration of 0.5-5 µM.
• Molecular Mass: 55 kDa
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -70 ºC as supplied.3 months, -70 ºC under sterile conditions after opening.

Gene Information

• Gene Name: MDM2 MDM2 proto-oncogene, E3 ubiquitin protein ligase [ Homo sapiens (human) ]
• Official Symbol: MDM2
• Synonyms: MDM2; MDM2 proto-oncogene, E3 ubiquitin protein ligase; HDMX; hdm2; ACTFS; E3 ubiquitin-protein ligase Mdm2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; double minute 2, human homolog of; p53-binding protein; oncoprotein Mdm2
• Gene ID: 4193
• mRNA Refseq: NM_001145337
• Protein Refseq: NP_001138809
• MIM: 164785
• UniProt ID: Q00987
• Chromosome Location: 12q14.3-q15
• Pathway: AKT phosphorylates targets in the cytosol; Adaptive Immune System; Cellular Senescence
• Function: enzyme binding; identical protein binding; ligase activity; p53 binding