Recombinant Human DNASE1 therapeutic protein

• Cat.No.: DNASE1-P001H
• Product Overview: Recombinant Human Deoxyribonuclease I therapeutic protein is a biosynthetic form of human deoxyribunuclease I (DNase I) enzyme. It is produced in genetically modified Chinese hamster ovary (CHO) cells using recombinant DNA technology. The 260-amino acid sequence of dornase alfa is identical to the endogenous human enzyme.

Specification

• Description: This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. The expression product is the active ingredient of Pulmozyme and Viscozyme.
• Source: CHO
• Species: Human
• Molecular Mass: 29.2kDa
• Protein length: 260 aa
• AA Sequence: LKIAAFNIQTFGETKMSNATLVSYI VQILSRYDIALVQEVRDSHLTAVGK LLDNLNQDAPDTYHYVVSEPL GRNSYKERYLFVYRPDQVSAVDSYY YDDGCEPCGNDTFNREPAIVRFFSR FTEVREFAIVPLHAAPGDAVA EIDALYDVYLDVQEKWGLEDVMLMG DFNAGCSYVRPSQWSSIRLWTSPTF QWLIPDSADTTATPTHCAYDR IVVAGMLLRGAVVPDSALPFNFQAA YGLSDQLAQAISDHYPVEVMLK
• Endotoxin: < 1.0 EU per μg of the protein
• Purity: >95%
• Storage: Can be stored at +4 centigrade short term (1-2weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoidrepeated freezing and thawing cycles.

Gene Information

• Gene Name: DNASE1 deoxyribonuclease I [ Homo sapiens ]
• Official Symbol: DNASE1
• Synonyms: DNASE1; deoxyribonuclease I; DNL1; deoxyribonuclease-1; Dornase alfa; DNase I, lysosomal; human urine deoxyribonuclease I; DRNI; FLJ38093; FLJ44902; DKFZp686H0155
• Gene ID: 1773
• mRNA Refseq: NM_005223
• Protein Refseq: NP_005214
• MIM: 125505
• UniProt ID: P24855
• Chromosome Location: 16p13.3
• Function: actin binding; deoxyribonuclease I activity; deoxyribonuclease activity; endonuclease activity; hydrolase activity; protein binding