| Description : |
This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Form : |
Freeze-dried powder |
| Molecular Mass : |
28kDa as determined by SDS-PAGE reducing conditions. |
| Protein length : |
Gly19~Ala259 |
| Endotoxin : |
<1.0EU per 1µg (determined by the LAL method). |
| Purity : |
>92% |
| Characteristic : |
The isoelectric point is 4.6. |
| Applications : |
SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling. |
| Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
| Storage buffer : |
PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300. |
| Reconstitution : |
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
