Recombinant B. thetaiotaomicron OGA, His-tagged OGA-85B

Recombinant B. thetaiotaomicron OGA, His-tagged

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Recombinant B. thetaiotaomicron OGA, His-tagged

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Cat.No. : OGA-85B
Product Overview : Recombinant B. thetaiotaomicron OGA(Gln22-Lys737), fused with an N-terminal Met and 6-His tag, was expressed in E. coli.
Description : Protein O-GlcNAcase (EC 3.2.1.169, OGA, glycoside hydrolase O-GlcNAcase, O-GlcNAcase, BtGH84, O-GlcNAc hydrolase) is an enzyme with system name (protein)-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase.
Source : E. coli
Species : B. thetaiotaomicron
Tag : His
Predicted N Terminal : Met
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl, Brij and Glycerol.
Bio-activity : Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-beta -D-glucosaminide (4-MU-GlcNAc) The specific activity is >3500 pmol/min/µg.
Molecular Mass : Predicted Molecular Mass:83 kDaSDS-PAGE:66-76 kDa, reducing conditions
Endotoxin : < 1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 ºC as supplied.3 months, -20 to -70 ºC under sterile conditions after opening.

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