Recombinant Human TIMP2, His-tagged

• Cat.No.: TIMP2-15H
• Product Overview: Recombinant human TIMP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 26.1 kDa (232aa), confirmed by MALDI-TOF
• Protein length: 27-220aa
• AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMFKGPEKDIEF IYTAPSSAVC GVSLDVGGKK EYLIAGKAEG DGKMHITLCD FIVPWDTLST TQKKSLNHRY QMGCECKITR CPMIPCYISS CS CSPVHPQQAF CNADVVIRAK AVSEKEVDSG NDIYGNPIKR IQYEIKQIKM PDECLWMDWV TEKNINGHQA KFFACIKRSD GSCAWYRGAA PPKQEFLDIE DP
• Purity: > 85% by SDS - PAGE
• Storage: Can be stored at +4ºC short term (1-2 weeks). For long term storage, aliquot and store at -20ºC or -70ºC. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TIMP2 TIMP metallopeptidase inhibitor 2 [ Homo sapiens (human) ]
• Official Symbol: TIMP2
• Synonyms: TIMP2; TIMP metallopeptidase inhibitor 2; DDC8; CSC-21K; metalloproteinase inhibitor 2; tissue inhibitor of metalloproteinases 2
• Gene ID: 7077
• mRNA Refseq: NM_003255
• Protein Refseq: NP_003246
• MIM: 188825
• UniProt ID: P16035
• Chromosome Location: 17q25
• Pathway: Activation of Matrix Metalloproteinases; Angiogenesis; Degradation of the extracellular matrix; Extracellular matrix organization
• Function: enzyme activator activity; integrin binding; metal ion binding; metalloendopeptidase inhibitor activity