Active Recombinant HIV-1 RT

• Cat.No.: RT-89H
• Product Overview: Recombinant HIV-1 Reverse Transcriptase was expressed in E. coli.

Specification

• Description: A Reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription. It is mainly associated with retroviruses. It should be noted however that also non-retroviruses use RT (for example, the hepatitis B virus, a member of the Hepadnaviridae, which are DsDNA-RT viruses, while retroviruses are ssRNA viruses). RT inhibitors are widely used as antiretroviral drugs. RT activities are also associated with the replication of chromosome ends (telomerase) and some mobile genetic elements (retrotransposons).
• Source: E. coli
• Species: HIV-1
• Form: 50% glycerol, 40 mM Tris-HCl (pH8.3), 50 mM NaCl, 5 mM MgCl2, 0.1% Triton X-100, 1 mM DTT
• Bio-activity: 5,000 units/ml
• Molecular Mass: It is composed of two subunits whose molecular weights are 66 kD and 51 kD, same as the those of the enzyme purified from AIDS virus particles.
• Purity: Over 90% by SDS-PAGE (CBB staining)
• Applications: 1) It is extremely effective for screening new specific inhibitors for HIV virus as a drug for treating AIDS.2) Generally, Gag and Env proteins are employed as antigens for detecting anti-HIV-1 antibody. However, by using this enzyme in combination as an antigen, the detection will be more sensitive.3) Reverse transcriptases are used in the first step of RT-PCR reaction for converting RNA to DNA. The HIV-1 reverse transcriptase can also be applied for RT-PCR method.4) Standards for SDS-PAGE, Western blotting, Dot blotting, ELISA
• Storage: Store at -20ºC
• Concentration: 0.5 mg/ml as measured by BCA method
• Storage Buffer: 50 mM Tris-HCl (pH 8.3), 10 mM MgCl2, 50mM KCl, 3 mM DTT, 0.1% Nonidet P-40, 20 ug/ml poly(rA)·oligo(dT)12-18, 0.5 mM dTTP ([3H]dTTP ,~1 x 105 cpm), and 10-50 units/ml reverse transcriptase.