Active Recombinant Rat DNA polymerase β

• Cat.No.: DNA polymerase β-136H
• Product Overview: This product is highly purified full-length rat DNA polymeraseβ overproduced in E. coli with high enzymatic activity without any tag attached. The enzyme has molecular mass of 38 kDa. The amino acid sequence of the rat enzyme has 86% identity to the human homolog.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: DNA polymeraseβ is a distributive polymerase involved in base excision repair which repairs damaged DNA by excising modified bases (oxidized, methylated, deaminated etc.)
• Form: 1.3 mg/ml in 50mM Tris-HCl pH7.6, 0.3M KCl, 0.1mM EDTA, 1mM DTT, 20% Glycerol
• Bio-activity: 90 unit/ul
• Purity: Over 95% pure by SDS-PAGE analysis
• Unit Definition: 1 unit of the enzyme activity incorporates 1 nanomole of dNTP into acid-insoluble fraction at 37℃ in 60 min.
• Applications: 1) For the studies on the mechanisms of base-excision repair of DNA damage 2) As a positive control of western blotting with anti-DNA polymeraseβ antibody
• Storage: Store at -20℃ ( long period, 70℃ )