Recombinant A. caninum NIF (Accession # AAA27789) Asn18-Leu274, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source :
Mammalian cells
Species :
A. caninum
Tag :
His
Predicted N Terminal :
Asn18
Form :
Lyophilized from a 0.2 µm filtered solution in PBS, NaCl, and DTT.
Bio-activity :
Measured by its ability to inhibit the adhesion of PMA-activated human neutrophils.The ED50 for this effect is typically 0.1-0.4 µg/mL.Also measured by its ability to bind Recombinant Human Integrin alpha M beta 2 in a functional ELISA.
Molecular Mass :
Recombinant A. caninum NIF, His-tagged has a calculated MW of 30 kDa. In SDS-PAGE migrates as 55-65 kDa, reducing conditions.
Purity :
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage :
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.