Recombinant A. caninum NIF, His-tagged NIF-157A

Recombinant A. caninum NIF, His-tagged


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Recombinant A. caninum NIF, His-tagged

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Cat.No. : NIF-157A
Product Overview : Recombinant A. caninum NIF (Accession # AAA27789) Asn18-Leu274, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : A. caninum
Tag : His
Predicted N Terminal : Asn18
Form : Lyophilized from a 0.2 µm filtered solution in PBS, NaCl, and DTT.
Bio-activity : Measured by its ability to inhibit the adhesion of PMA-activated human neutrophils.The ED50 for this effect is typically 0.1-0.4 µg/mL.Also measured by its ability to bind Recombinant Human Integrin alpha M beta 2 in a functional ELISA.
Molecular Mass : Recombinant A. caninum NIF, His-tagged has a calculated MW of 30 kDa. In SDS-PAGE migrates as 55-65 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

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