Active Recombinant A. caninum NIF, His-tagged

• Cat.No.: NIF-157A
• Product Overview: Recombinant A. caninum NIF (Accession # AAA27789) Asn18-Leu274, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: A.caninum
• Source: Mammalian Cells
• Tag: His
• Protein Length: 18-274 a.a.
• Predicted N Terminal: Asn18
• Form: Lyophilized from a 0.2 µm filtered solution in PBS, NaCl, and DTT.
• Bio-activity: Measured by its ability to inhibit the adhesion of PMA-activated human neutrophils.The ED50 for this effect is typically 0.1-0.4 µg/mL.Also measured by its ability to bind Recombinant Human Integrin alpha M beta 2 in a functional ELISA.
• Molecular Mass: Recombinant A. caninum NIF, His-tagged has a calculated MW of 30 kDa. In SDS-PAGE migrates as 55-65 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).