| Cat.No.: | NIF-157A |
| Product Overview: | Recombinant A. caninum NIF (Accession # AAA27789) Asn18-Leu274, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived. |
| Source: | NSO cells |
| Species: | A. caninum |
| Tag: | His |
| Predicted N Terminal: | Asn18 |
| Form: | Lyophilized from a 0.2 µm filtered solution in PBS, NaCl, and DTT. |
| Bio-activity: | Measured by its ability to inhibit the adhesion of PMA-activated human neutrophils.The ED50 for this effect is typically 0.1-0.4 µg/mL.Also measured by its ability to bind Recombinant Human Integrin alpha M beta 2 in a functional ELISA. |
| Molecular Mass: | Recombinant A. caninum NIF, His-tagged has a calculated MW of 30 kDa. In SDS-PAGE migrates as 55-65 kDa, reducing conditions. |
| Purity: | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Storage: | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
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