Active Recombinant A. caninum NIF, His-tagged
Cat.No. : | NIF-157A |
Product Overview : | Recombinant A. caninum NIF (Accession # AAA27789) Asn18-Leu274, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived. |
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Species : | A.caninum |
Source : | Mammalian Cells |
Tag : | His |
Protein Length : | 18-274 a.a. |
Predicted N Terminal : | Asn18 |
Form : | Lyophilized from a 0.2 µm filtered solution in PBS, NaCl, and DTT. |
Bio-activity : | Measured by its ability to inhibit the adhesion of PMA-activated human neutrophils.The ED50 for this effect is typically 0.1-0.4 µg/mL.Also measured by its ability to bind Recombinant Human Integrin alpha M beta 2 in a functional ELISA. |
Molecular Mass : | Recombinant A. caninum NIF, His-tagged has a calculated MW of 30 kDa. In SDS-PAGE migrates as 55-65 kDa, reducing conditions. |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
◆ Recombinant Proteins | ||
NIF-157A | Active Recombinant A. caninum NIF, His-tagged | +Inquiry |
Not For Human Consumption!
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