Active Recombinant Human GUSB, His-tagged

• Cat.No.: GUSB-179H
• Product Overview: Recombinant Human GUSB (Accession # AAA52561) Met1-Thr651, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Leu23
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to hydrolyze 4-methylumbelliferyl-beta -D-glucuronide.The specific activity is >4,000 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
• Molecular Mass: Recombinant Human GUSB, His-tagged has a calculated MW of 73 kDa. In SDS-PAGE migrates as 75-85 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: GUSB glucuronidase, beta [ Homo sapiens ]
• Official Symbol: GUSB
• Synonyms: GUSB; glucuronidase, beta; beta-glucuronidase; beta-G1; beta-D-glucuronidase; BG; MPS7; FLJ39445
• Gene ID: 2990
• mRNA Refseq: NM_000181
• Protein Refseq: NP_000172
• MIM: 611499
• UniProt ID: P08236
• Chromosome Location: 7q11.21