Active Recombinant Human SERPINA4, His-tagged

• Cat.No.: SERPINA4-185H
• Product Overview: Recombinant Human SERPINA4 (Accession # AAA59454) Gln21-Pro427, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: No results obtained: Gln21 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
• Bio-activity: Measured by its ability to inhibit KLK1 cleavage of a fluorogenic peptide substrate, Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).The IC50 value is <40 nM, as measured under the described conditions.
• Molecular Mass: Recombinant Human SERPINA4, His-tagged has a calculated MW of 48 kDa. In SDS-PAGE migrates as 57 kDa and 61 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SERPINA4 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 [ Homo sapiens ]
• Official Symbol: SERPINA4
• Synonyms: KAL; KST; PI4; KLST; PI-4; kallistatin
• Gene ID: 5267
• mRNA Refseq: NM_006215.2
• Protein Refseq: NP_006206.2
• MIM: 147935
• UniProt ID: P29622
• Chromosome Location: 14q31-q32.1