Active Recombinant Human FRZB, His-tagged

• Cat.No.: FRZB-208H
• Product Overview: Recombinant Human FRZB (Accession # AAB51298) Ala33-Asn325, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala33
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 1 - 4 µg/mL.Optimal concentrations should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human FRZB, His-tagged has a calculated MW of 35 kDa. In SDS-PAGE migrates as 38 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: FRZB frizzled-related protein [ Homo sapiens ]
• Official Symbol: FRZB
• Synonyms: FRZB; frizzled-related protein; secreted frizzled-related protein 3; FRE; FRITZ; FRP 3; FRZB 1; FRZB PEN; FRZB1; FZRB; hFIZ; SFRP3; SRFP3; sFRP-3; frezzled; frizzled homolog-related; frizzled-related protein 1; OS1; FRP-3; FRZB-1; FRZB-PEN
• Gene ID: 2487
• mRNA Refseq: NM_001463
• Protein Refseq: NP_001454
• MIM: 605083
• UniProt ID: Q92765
• Chromosome Location: 2qter