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Recombinant Human FRZB, His-tagged

Cat.No.: FRZB-208H
Product Overview: Recombinant Human FRZB (Accession # AAB51298) Ala33-Asn325, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Human
Tag: His
Predicted N Terminal: Ala33
Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity: Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 1 - 4 µg/mL.Optimal concentrations should be determined by each laboratory for each application.
Molecular Mass: Recombinant Human FRZB, His-tagged has a calculated MW of 35 kDa. In SDS-PAGE migrates as 38 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: FRZB frizzled-related protein [ Homo sapiens ]
Official Symbol: FRZB
Synonyms: FRZB; frizzled-related protein; secreted frizzled-related protein 3; FRE; FRITZ; FRP 3; FRZB 1; FRZB PEN; FRZB1; FZRB; hFIZ; SFRP3; SRFP3; sFRP-3; frezzled; frizzled homolog-related; frizzled-related protein 1; OS1; FRP-3; FRZB-1; FRZB-PEN;
Gene ID: 2487
mRNA Refseq: NM_001463
Protein Refseq: NP_001454
MIM: 605083
UniProt ID: Q92765
Chromosome Location: 2qter

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