Active Recombinant Human SFRP2

• Cat.No.: SFRP2-299H
• Product Overview: Recombinant Human SFRP2 (Accession # AAH08666) Leu25-Cys295 was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Predicted N Terminal: Leu25 (major) & Phe26 (minor)
• Form: Lyophilized from a 0.2 µm filtered solution in PBS and NaCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to synergize with Wnt-3a to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is typically 10-40 ng/mL in the presence of 20 ng/mL of Recombinant Mouse Wnt-3a .
• Molecular Mass: Recombinant Human SFRP2 has a calculated MW of 31 kDa. In SDS-PAGE migrates as 34-36 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 200 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: SFRP2 secreted frizzled-related protein 2 [ Homo sapiens ]
• Official Symbol: SFRP2
• Synonyms: SFRP2; secreted frizzled-related protein 2; FRP 2; SARP1; SDF 5; SARP-1; sFRP-2; secreted apoptosis related protein 1; secreted apoptosis-related protein 1; FRP-2; SDF-5
• Gene ID: 6423
• mRNA Refseq: NM_003013
• Protein Refseq: NP_003004
• MIM: 604157
• UniProt ID: Q96HF1
• Chromosome Location: 4q31.3