Recombinant Human SFRP2 SFRP2-299H

Recombinant Human SFRP2

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Recombinant Human SFRP2

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Cat.No. : SFRP2-299H
Product Overview : Recombinant Human SFRP2 (Accession # AAH08666) Leu25-Cys295 was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Human
Predicted N Terminal : Leu25 (major) & Phe26 (minor)
Form : Lyophilized from a 0.2 µm filtered solution in PBS and NaCl with BSA as a carrier protein.
Bio-activity : Measured by its ability to synergize with Wnt-3a to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is typically 10-40 ng/mL in the presence of 20 ng/mL of Recombinant Mouse Wnt-3a .
Molecular Mass : Recombinant Human SFRP2 has a calculated MW of 31 kDa. In SDS-PAGE migrates as 34-36 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 200 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : SFRP2 secreted frizzled-related protein 2 [ Homo sapiens ]
Official Symbol : SFRP2
Synonyms : SFRP2; secreted frizzled-related protein 2; FRP 2; SARP1; SDF 5; SARP-1; sFRP-2; secreted apoptosis related protein 1; secreted apoptosis-related protein 1; FRP-2; SDF-5;
Gene ID : 6423
mRNA Refseq : NM_003013
Protein Refseq : NP_003004
MIM : 604157
UniProt ID : Q96HF1
Chromosome Location : 4q31.3

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