Recombinant Human ECM1, His-tagged ECM1-315H

Recombinant Human ECM1, His-tagged

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Recombinant Human ECM1, His-tagged

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Cat.No. : ECM1-315H
Product Overview : Recombinant Human ECM1 (Accession # AAH23505) Ala20-Glu540, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Human
Tag : His
Predicted N Terminal : Ala20
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells.When 5 x 10^4 cells/well are added to rhECM-1/His coated plates (10 µg/mL with 100 µL/well), approximately 30 - 65% will adhere after 30 minutes at 37° C. Optimal concentration depends on cell type as well as the application or research objective.
Molecular Mass : Recombinant Human ECM1, His-tagged has a calculated MW of 59.7 kDa. In SDS-PAGE migrates as 77-87 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : ECM1 extracellular matrix protein 1 [ Homo sapiens ]
Official Symbol : ECM1
Synonyms : ECM1; extracellular matrix protein 1; secretory component p85;
Gene ID : 1893
mRNA Refseq : NM_001202858
Protein Refseq : NP_001189787
MIM : 602201
UniProt ID : Q16610
Chromosome Location : 1q21

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