"ECM1" Related Products

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Recombinant Human ECM1, His-tagged

Cat.No.: ECM1-315H
Product Overview: Recombinant Human ECM1 (Accession # AAH23505) Ala20-Glu540, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source: NSO cells
Species: Human
Tag: His
Predicted N Terminal: Ala20
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells.When 5 x 10^4 cells/well are added to rhECM-1/His coated plates (10 µg/mL with 100 µL/well), approximately 30 - 65% will adhere after 30 minutes at 37° C. Optimal concentration depends on cell type as well as the application or research objective.
Molecular Mass: Recombinant Human ECM1, His-tagged has a calculated MW of 59.7 kDa. In SDS-PAGE migrates as 77-87 kDa, reducing conditions.
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: ECM1 extracellular matrix protein 1 [ Homo sapiens ]
Official Symbol: ECM1
Synonyms: ECM1; extracellular matrix protein 1; secretory component p85;
Gene ID: 1893
mRNA Refseq: NM_001202858
Protein Refseq: NP_001189787
MIM: 602201
UniProt ID: Q16610
Chromosome Location: 1q21

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