Active Recombinant Human SMOC2, His-tagged

• Cat.No.: SMOC2-332H
• Product Overview: Recombinant Human SMOC2 (Accession # AAH47583) Gln22-Gly446, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Human
• Tag: His
• Predicted N Terminal: No results obtained: Gln22 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.When 5 x 10^4 cells/well are added to rhSMOC-2 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED50 for this effect is typically 0.4 - 1.6 µg/mL.
• Molecular Mass: Recombinant Human SMOC2, His-tagged has a calculated MW of 48.3 kDa. In SDS-PAGE migrates as 58-66 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SMOC2 SPARC related modular calcium binding 2 [ Homo sapiens ]
• Official Symbol: SMOC2
• Synonyms: SMOC2; SPARC related modular calcium binding 2; SPARC-related modular calcium-binding protein 2; SMAP2; SMAP-2; SMOC-2; smooth muscle associated protein 2; smooth muscle-associated protein 2; secreted modular calcium-binding protein 2; thyroglobulin type-1 repeat containing protein; DTDP1; MST117; MSTP117; MSTP140; bA37D8.1; bA270C4A.1; dJ421D16.1; RP11-270C4__A.1
• Gene ID: 64094
• mRNA Refseq: NM_001166412
• Protein Refseq: NP_001159884
• MIM: 607223
• UniProt ID: Q9H3U7
• Chromosome Location: 6q27