Active Recombinant Human ANGPTL5, His-tagged

• Cat.No.: ANGPTL5-333H
• Product Overview: Recombinant Human ANGPTL5 (Accession # AAH49170) Met1-Lys388, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Human
• Tag: His
• Predicted N Terminal: Val23 & Asn26
• Form: Lyophilized from a 0.2 µm filtered solution in PBS and NaCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand , Recombinant Mouse Thrombopoietin/Tpo , and Recombinant Mouse Flt-3 Ligand .The ED50 for this effect is typically 70 - 280 ng/mL in the presence of a cross-linking mouse polyHistidine Antibody.
• Molecular Mass: Recombinant Human ANGPTL5, His-tagged has a calculated MW of 42.3 kDa. In SDS-PAGE migrates as 50-60 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in PBS.

Gene Information

• Gene Name: ANGPTL5 angiopoietin-like 5 [ Homo sapiens ]
• Official Symbol: ANGPTL5
• Synonyms: ANGPTL5; angiopoietin-like 5; angiopoietin-related protein 5; angiopoietin-like protein 5
• Gene ID: 253935
• mRNA Refseq: NM_178127
• Protein Refseq: NP_835228
• MIM: 607666
• UniProt ID: Q86XS5
• Chromosome Location: 11q22.2