Active Recombinant C. perfringens NAGA, Met&His-tagged

• Cat.No.: NAGA-401C
• Product Overview: Recombinant C. perfringens NAGA (Accession # AAM55479) Lys2-Lys619, fused with an N-terminal Met and 6-His tag, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: C. perfringens
• Tag: &His
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to cleave alpha -N-acetylgalactosaminyl from 4-Nitrophenyl N-acetyl-alpha -D-galactosaminide.The specific activity is >5,000 pmol/min/ug, as measured under the described conditions.
• Molecular Mass: Recombinant C. perfringens NAGA, Met&His-tagged has a calculated MW of 75 kDa. In SDS-PAGE migrates as 65 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).