Active Recombinant Human GPR56, His-tagged

• Cat.No.: GPR56-409H
• Product Overview: Recombinant Human GPR56 (Accession # AAP35975.1) Arg26-Val342, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Human
• Tag: His
• Predicted N Terminal: Arg26
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to enhance the adhesion of T98G human glioblastoma cells to human Fibronectin.When 4 x 10^4 cells/well are added to plates coated with human Fibronectin (0.1 µg/mL) and rhGPR56 (10 µg/well, 100 µL/well) for 45 minutes at 37° C there is a 2 - 4 fold increase in adhesion to human fibronectin induced by the presence of rhGPR56.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human GPR56, His-tagged has a calculated MW of 36.8 kDa. In SDS-PAGE migrates as 65-85 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: GPR56 G protein-coupled receptor 56 [ Homo sapiens ]
• Official Symbol: GPR56
• Synonyms: GPR56; G protein-coupled receptor 56; G-protein coupled receptor 56; TM7LN4; TM7XN1; 7-transmembrane protein with no EGF-like N-terminal domains-1; BFPP; DKFZp781L1398
• Gene ID: 9289
• mRNA Refseq: NM_001145770
• Protein Refseq: NP_001139242
• MIM: 604110
• UniProt ID: Q9Y653
• Chromosome Location: 16q13