Recombinant Human CSPG5, His-tagged CSPG5-416H

Recombinant Human CSPG5, His-tagged

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Recombinant Human CSPG5, His-tagged

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Cat.No. : CSPG5-416H
Product Overview : Recombinant Human CSPG5 (Accession # AAQ04774) Val31-Gln420, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO cells
Species : Human
Tag : His
Predicted N Terminal : Val31
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by its ability to inhibit Recombinant Human Midkine induced neurite outgrowth in E16-E18 rat embryonic cortical neurons.When 5 x 10^4 neurons per well are added to a plate pre-incubated with serial dilutions of rhNeuroglycan-C and 1 µg/mL of rhMidkine, the neurite outgrowth promotion is significantly inhibited in a dose dependent manner with an effect typically at 0.6 - 1.2 µg/mL.
Molecular Mass : Recombinant Human CSPG5, His-tagged has a calculated MW of 41.9 kDa. In SDS-PAGE migrates as 80-100 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : CSPG5 chondroitin sulfate proteoglycan 5 (neuroglycan C) [ Homo sapiens ]
Official Symbol : CSPG5
Synonyms : CSPG5; chondroitin sulfate proteoglycan 5 (neuroglycan C); chondroitin sulfate proteoglycan 5; NGC; acidic leucine-rich EGF-like domain-containing brain protein; MGC44034;
Gene ID : 10675
mRNA Refseq : NM_001206942
Protein Refseq : NP_001193871
MIM : 606775
UniProt ID : O95196
Chromosome Location : 3p21.3

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