Active Recombinant Human MANF, His-tagged

• Cat.No.: MANF-429H
• Product Overview: Recombinant Human MANF (Accession # ACE87065) Leu22-Leu179, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Leu22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to support the survival and stimulate neurite outgrowth of rat embryonic cortical neurons.The ED50 for this effect is typically 0.7-2.8 µg/mL.
• Molecular Mass: Recombinant Human MANF, His-tagged has a calculated MW of 19.0 kDa. In SDS-PAGE migrates as 20.4 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: MANF mesencephalic astrocyte-derived neurotrophic factor [ Homo sapiens ]
• Official Symbol: MANF
• Synonyms: MANF; mesencephalic astrocyte-derived neurotrophic factor; arginine rich, mutated in early stage tumors , ARMET; ARP; arginine-rich, mutated in early stage tumors; ARMET; MGC142148; MGC142150
• Gene ID: 7873
• mRNA Refseq: NM_006010
• Protein Refseq: NP_006001
• MIM: 601916
• UniProt ID: P55145
• Chromosome Location: 3p21.1