Recombinant Human MANF, His-tagged MANF-429H

Recombinant Human MANF, His-tagged


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Recombinant Human MANF, His-tagged

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Cat.No. : MANF-429H
Product Overview : Recombinant Human MANF (Accession # ACE87065) Leu22-Leu179, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Human
Tag : His
Predicted N Terminal : Leu22
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by its ability to support the survival and stimulate neurite outgrowth of rat embryonic cortical neurons.The ED50 for this effect is typically 0.7-2.8 µg/mL.
Molecular Mass : Recombinant Human MANF, His-tagged has a calculated MW of 19.0 kDa. In SDS-PAGE migrates as 20.4 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : MANF mesencephalic astrocyte-derived neurotrophic factor [ Homo sapiens ]
Official Symbol : MANF
Synonyms : MANF; mesencephalic astrocyte-derived neurotrophic factor; arginine rich, mutated in early stage tumors , ARMET; ARP; arginine-rich, mutated in early stage tumors; ARMET; MGC142148; MGC142150;
Gene ID : 7873
mRNA Refseq : NM_006010
Protein Refseq : NP_006001
MIM : 601916
UniProt ID : P55145
Chromosome Location : 3p21.1

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