Active Recombinant Mouse AF251705, Fc Chimera

• Cat.No.: AF251705-457M
• Product Overview: Recombinant Mouse AF251705 (Accession # BAC80269) was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Mouse
• Tag: Fc Chimera
• Predicted N Terminal: Cys22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and serially diluted Recombinant Mouse CD300c/LMIR2 Fc Chimera. The ED50 for this effect is typically 0.6-3 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse AF251705, Fc Chimera has a calculated MW of 46 kDa (monomer). In SDS-PAGE migrates as 55-65 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: AF251705 cDNA sequence AF251705 [ Mus musculus (house mouse) ]
• Official Symbol: AF251705
• Synonyms: AF251705; cDNA sequence AF251705; CLM4; DIgR1; Igsf7; Lmir2; Cd300c; Cd300d; MAIR-II; CMRF35-like molecule; CLM; Cd300C antigen; Cd300D antigen; MAIR-2; dendritic cell-derived Ig-like receptor 1; dendritic cell-derived immunoglobulin receptor 1; immunoglobulin superfamily member 7; immunoglobulin superfamily, member 7; leukocyte mono-Ig-like receptor 2; myeloid-associated immunoglobulin-like receptor 2; myeloid-associated immunoglobulin-like receptor II
• Gene ID: 140497
• mRNA Refseq: NM_134158
• Protein Refseq: NP_598919
• UniProt ID: Q7TSN2
• Chromosome Location: 11 E2; 11 80.57 cM