Recombinant Human EPCAM protein, Fc-tagged EPCAM-475H

Recombinant Human EPCAM protein, Fc-tagged

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Recombinant Human EPCAM protein, Fc-tagged

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Cat.No. : EPCAM-475H
Product Overview : Recombinant Human EPCAM(Gln24 - Lys265) fused with Fc tag at C-terminal was expressed in NS0.
Source : NS0
Species : Human
Tag : Fc
Predicted N Terminal : Gln24
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.When 5 x 10^4 cells/well are added to recombinant human EpCAM/Fc Chimera and human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 centigrade. The ED50 for this effect is typically 0.7-2.8 μg/mL. Measured by its binding ability in a functional ELISA. The activity of this protein is measured in a homophilic binding assay.
Molecular Mass : Predicted Molecular Mass: 54 (monomer) kDa;SDS-PAGE: 60-70 kDa, reducing conditions
Protein length : Gln24 - Lys265
Endotoxin : <0.10 EU per 1 μg of the protein by the LAL method.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100 μg/mL in sterile PBS.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Gene Name : EPCAM epithelial cell adhesion molecule [ Homo sapiens ]
Official Symbol : EPCAM
Synonyms : EPCAM; epithelial cell adhesion molecule; antigen identified by monoclonal AUA1 , M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1; 17 1A; 323/A3; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; GA733 2; HEA125; KS1/4; KSA; Ly74; MH99; MK 1; MOC31; TACST 1; TROP1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); M4S1; MK-1; DIAR5; EGP-2; MIC18; EGP314; HNPCC8; TACSTD1; GA733-2;
Gene ID : 4072
mRNA Refseq : NM_002354
Protein Refseq : NP_002345
MIM : 185535
UniProt ID : P16422
Chromosome Location : 2p21
Function : protein binding;

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