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Recombinant Human CTSH, His-tagged

Cat.No.: CTSH-478H
Product Overview: Recombinant Human CTSH (Accession # CAA34734) Ala23-Val335, fused with a C-terminal 10-His tag., was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source: CHO cells
Species: Human
Tag: His
Predicted N Terminal: Ala23
Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC). The specific activity is >750 pmol/min/µg, as measured under the described conditions.
Molecular Mass: Recombinant Human CTSH, His-tagged has a calculated MW of 37 kDa. In SDS-PAGE migrates as 38-43 kDa, reducing conditions.
Purity: >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: CTSH cathepsin H [ Homo sapiens ]
Official Symbol: CTSH
Synonyms: CTSH; cathepsin H; CPSB; pro-cathepsin H; ACC 4; ACC 5; aleurain; cathepsin B3; cathepsin BA; N-benzoylarginine-beta-naphthylamide hydrolase; ACC-4; ACC-5; minichain; MGC1519; DKFZp686B24257;
Gene ID: 1512
mRNA Refseq: NM_004390
Protein Refseq: NP_004381
MIM: 116820
UniProt ID: P09668
Chromosome Location: 15q25.1

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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