Active Recombinant Human CTSH, His-tagged

• Cat.No.: CTSH-478H
• Product Overview: Recombinant Human CTSH (Accession # CAA34734) Ala23-Val335, fused with a C-terminal 10-His tag., was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala23
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC). The specific activity is >750 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human CTSH, His-tagged has a calculated MW of 37 kDa. In SDS-PAGE migrates as 38-43 kDa, reducing conditions.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CTSH cathepsin H [ Homo sapiens ]
• Official Symbol: CTSH
• Synonyms: CTSH; cathepsin H; CPSB; pro-cathepsin H; ACC 4; ACC 5; aleurain; cathepsin B3; cathepsin BA; N-benzoylarginine-beta-naphthylamide hydrolase; ACC-4; ACC-5; minichain; MGC1519; DKFZp686B24257
• Gene ID: 1512
• mRNA Refseq: NM_004390
• Protein Refseq: NP_004381
• MIM: 116820
• UniProt ID: P09668
• Chromosome Location: 15q25.1