Active Recombinant Human LBP, His-tagged
Cat.No. : | LBP-505H |
Product Overview : | Recombinant Human LBP (Accession # CAA67226) Ala26-Val481, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived. |
- Specification
- Gene Information
- Related Products
Source : | Mammalian cells |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Ala26 |
Form : | Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein. |
Bio-activity : | Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells.The ED50 for this effect is typically 0.25 - 1.5 ng/mL. |
Molecular Mass : | Recombinant Human LBP, His-tagged has a calculated MW of 51.7 kDa. In SDS-PAGE migrates as 60-65 kDa, reducing conditions. |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Reconstitution : | Reconstitute at 100 µg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
Gene Name : | LBP lipopolysaccharide binding protein [ Homo sapiens ] |
Official Symbol : | LBP |
Synonyms : | LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; BPI fold containing family D; member 2; BPIFD2; LPS-binding protein; BPI fold containing family D, member 2; MGC22233; |
Gene ID : | 3929 |
mRNA Refseq : | NM_004139 |
Protein Refseq : | NP_004130 |
MIM : | 151990 |
UniProt ID : | P18428 |
Chromosome Location : | 20q11.23 |
Products Types
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◆ Lysates | ||
LBP-1853HCL | Recombinant Human LBP cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionIf LPS's chemical spatial configuration prevents close contact with TLR4, it exhibits an antagonistic effect, inhibiting the cellular response.
LPS on the LBP-LPS complex, upon close physical contact with TLR4 on cells, induces TLR4 receptor dimerization or multimerization. This activates various transcription factors, leading to the expression of inflammatory mediators and cytokines.
For cells without membrane CD14 receptors (e.g., endothelial and epithelial cells), a soluble CD14 (sCD14)-LBP-LPS ternary complex forms, activating TLR4 and inducing various biological effects.
LBP stands for Lipopolysaccharide-Binding Protein.
LBP has a high affinity for the lipid A portion in the structure of Lipopolysaccharide (LPS) and forms the LPS-LBP complex.
Liver cells, similar to macrophages, express mCD14 and TLR4. In the absence of CD14 receptors, LPS can still bind to TLR4, but with relatively weaker intensity.
The LBP-LPS complex can transfer LPS to membrane CD14 (mCD14) receptors on cells like monocytes, macrophages, and neutrophils.
Customer Reviews (3)
Write a reviewWe appreciated the product's efforts to minimize its environmental footprint through sustainable manufacturing practices.
Its adaptability to various sample types and conditions made it a valuable asset for our diverse projects.
The product's versatility allowed us to apply it to a wide range of experimental techniques and research areas.
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