Active Recombinant Human ECE2, His&Val-tagged

• Cat.No.: ECE2-529H
• Product Overview: Recombinant Human ECE2 (Accession # EAW78275) Gly104-Trp787, fused with an N-terminal 10-His tag and Val, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His&Val
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and ZnCl2.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH .The specific activity is > 250 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human ECE2, His&Val-tagged has a calculated MW of 79 kDa. In SDS-PAGE migrates as 113 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ECE2 endothelin converting enzyme 2 [ Homo sapiens ]
• Official Symbol: ECE2
• Synonyms: ECE2; endothelin converting enzyme 2; endothelin-converting enzyme 2; KIAA0604; MGC2408; ECE-2; MGC17664; MGC78487
• Gene ID: 9718
• mRNA Refseq: NM_001037324
• Protein Refseq: NP_001032401
• MIM: 610145
• UniProt ID: O60344
• Chromosome Location: 3q27.1